Cysteine Protease Bleomycin Hydrolase Genomic Structure and Genetic Mapping of the Human Neutral
نویسندگان
چکیده
Bleomycin hydrolase (BH) is the only known eukaryotic enzyme that inactivates the widely used antineoplastic agent bleomycin (BLM) and is a primary candidate gene for protection against lethal BLM-induced pul monary fibrosis and for BLM resistance in tumors. Human B!! was found to exist as a single gene that was mapped to chromosome 17 using National Institute of General Medical Sciences human/rodent hybrid mapping panels and localized to 17q11.1—11.2 by linkage analysis using the Centre d'Etude du Polymorphisme Humain reference database. The human BH gene consisted of 11 exons ranging in size from 69—198 bp separated by introns ofapproximately 1kb, reflectingthe archetypal genomicstructure of the cysteine protease family. A polymorphic site was identified in the eleventh exon at bp 1450 encodIng either valine or Isoleucine. These fmdings provide essential tools requlred to define the role of RH in BLM-induced pulmonary fibrosis and BLM resistance in tumors.
منابع مشابه
Genomic structure and genetic mapping of the human neutral cysteine protease bleomycin hydrolase.
Bleomycin hydrolase (BH) is the only known eukaryotic enzyme that inactivates the widely used antineoplastic agent bleomycin (BLM) and is a primary candidate gene for protection against lethal BLM-induced pulmonary fibrosis and for BLM resistance in tumors. Human BH was found to exist as a single gene that was mapped to chromosome 17 using National Institute of General Medical Sciences human/ro...
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